果蝇zeste基因增强子2和信号转导与转录激活子3在人胃癌组织中的表达及意义

Expressions and significance of enhancer of zeste homology 2 and signal transducer and activator of transcription 3 in gastric cancer tissues

    摘要
  • 摘要: 目的
    检测胃癌组织标本中果蝇zeste基因增强子2(EZH2)和信号转导与转录激活子3(STAT3)的表达情况，并分析其与临床病理学特征以及患者预后关系。
    方法
    回顾性分析1999年5月至2000年4月北京大学人民医院收治的67例胃癌患者的临床资料。连续收集患者手术切除的胃癌组织及癌旁组织(距肿瘤边缘>5 cm)，用4%甲醛固定，常规石蜡包埋后，制作组织芯片，通过免疫组织化学染色法检测组织中EZH2和STAT3的表达情况，并分析其与临床病理学特征以及患者预后关系。胃癌组织中EZH2、STAT3的蛋白表达水平与临床病理指标之间的关系用配对 χ 2 检验，KaplanMeier法绘制胃癌患者的生存曲线，Logrank检验生存率，COX比例风险模型分析胃癌各临床病理指标与预后之间的关系。
    结果胃癌 组织中EZH2和STAT3蛋白的高表达率分别为73.1%(49/67)和67.2%(45/67)，显著高于癌旁组织中的32.8%(22/67)和37.3%(25/67)，两者比较，差异有统计学意义( χ 2 STAT3表达、淋巴结转移和远处转移是影响胃癌患者生存的危险因素( RR=2.136，3.452，3.179，8.341，11.773，6.873，P< 0.05)。多因素分析结果显示：TNM分期、STAT3表达水平是影响胃癌预后的独立危险因素( RR= 2.453，7.535，P< 0.05)。
    结论
    胃癌中EZH2及STAT3表达有显著相关性；其联合表达模式可能是判断胃癌TNM分期及患者预后的肿瘤分子标志物。

     

    Abstract: Objective
    To detect the expressions of enhancer of zeste homology 2 (EZH2) and signal transducer and activator of transcription 3 (STAT3) in gastric cancer tissues, and analyze the relationship between their expressions and clinicopathological factors and prognosis of patients.
    Methods
    The clinical data of 67 patients with gastric cancer who were admitted to the People′s Hospital of Peking University from May 1999 to April 2000 were retrospectively analyzed. Gastric cancer tissues and adjacent normal tissues were harvested and fixed in 4% formaldehyde, and then to make the tissue chips. The protein expressions of EZH2 and STAT3 were detected by immunohistochemical staining, and the relationship between the expressions of EZH2 and STAT3 and the prognosis of patients was analyzed. The correlation between the protein expressions of EZH2 and STAT3 〖HJ*6〗in the gastric and colorectal tissues and the clinicopathological parameters was analyzed using the paired chi square test. The survival curve was drawn by Kaplan Meier method, and the survival rate was analyzed by using the Log rank test. The relationship between the clinicopathological factors and the prognosis of patients was analyzed using the COX proportional hazard model.
    Results
    The positive protein expression rates of EZH2 and STAT3 in the gastric cancer tissues were 73.1%(49/67) and 67.2% (45/67), which were significantly higher than 32.8%(22/67) and 37.3%(25/67) in the adjacent normal tissues ( χ 2 ( RR=2.136, 3.452, 3.179, 8.341, 11.773, 6.873, P <0.05). The results of multivariate analysis showed that TNM staging and STAT3 expression were independent factors influencing the prognosis of patients with gastric cancer ( RR=2.453, 7.535, P <0.05).
    Conclusions
    There is significant correlation between EZH2 and STAT3 protein expressions in gastric cancer tissues, and co expression of EZH2 and STAT3 is associated with the TNM staging and the prognosis of the patients with gastric cancer.

     

    • HTML全文
    • 参考文献(0)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回